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මෙම කවුළුව, ඹබව ඊළගට රැගෙන යන වෙබ් පිටුව පිළිබද ඉගියක් සපයනු ඇත

About the Research Contributors Main List

Pyramiding of resistant genes into local rice varieties for Bacterial Leaf Blight (BLB), Gall Midge (GM) and Brown Planthopper (BPH) using Marker Assisted Selection (MAS)

Brown Planthopper (BPH) and Gall Midge (GM) are two important pests and Bacterial Leaf Blight (BLB) is one of the major diseases of rice in Sri Lanka. Development of resistant varieties is the most suitable solution to overcome these problems. However, some of the resistant rice varieties released in the recent past has become susceptible to targeted pests and diseases. Immergence of virulent populations indicates a development of new biotype(s)/pathotypes. Therefore, pyramiding resistant genes of BPH, GM and BLB will reduce out-breaks and facilitate durable resistance.

Bacterial Leaf Blight Gall Midge Brown Planthopper

To address these issues, Department of Agriculture is involved in Sri Lanka – United States Department of Agriculture (SL-USDA) project Pyramiding of Genes for Resistance to Brown Planthopper (BPH), Bacterial Leaf Blight (BLB) and Gall Midge (GM) into new improved Rice Varieties” to develop varieties with durable resistance to BPH, GM and BLB by pyramiding major genes from different sources. The advanced breeding lines having field resistance to BPH, GM and BLB resistance were subjected to genetic analysis using molecular techniques to assist the breeding programs with Marker Aided Selection (MAS). Simultaneously, identification of new biotypes / pathotypes is also being done as a part of the project activity.

Research Findings


Brown Planthopper

Gall Midge

Eg. The GM primer F10 cannot be used to identify the presence of resistant gene GM2 in tested rice samples. (Dissanayake et. al., 2005)

BLB

Contributors


Dr. K.K.S. Fernando (Principal Investigator- Biotechnology)
Dr. R.M.T. Rajapakse (Co-Investigator-Breeding & Pathology)
Dr.W.L.G. Samarasinghe (Research Officer, Biotechnology)
Mr.A.H. Gunadasa (Research Officer, Plant Breeding)
Mr.A.M.T.Abayaywickrama (Research Officer)
Ms. D.M.K.K. Dissanayake (Programme Assistant)
Mr. J.H.B.H. Bandara (Graduate Research Assistant)
Mr. E.M. D. S. B. Ekanayake (Graduate Research Assistant)
Ms. S.I. Samararatne (Graduate Research Assistant)
Ms. S.K.Hewakapuge (Graduate Research Assistant)
Ms. G.K. Wanigasekara (Technical Assistant)
Mr. Herath (Technical Assistant)

Reference:

  1. D.M.K.K. Dissanayake, K.K.S. Fernando and J.M.R.S. Bandara. 2005. Molecular Screening of Selected Rice Varieties with Specific Markers for Gm2 and Gm4 (t) Gall Midge Resistant Genes, Tropical Agricultural Research Vol. 17: 48 - 57.
  2. K.K.S. Fernando, J.H.B.H. Bandara, R.M.T. Rajapakse, V.A. Sumanasinghe. 2005. Detection of Genetic variability and Pathagenicity of Xanthomonas oryzae p.v. Oryzae: Causal organism of Bacterial leaf Blight in rice. Annals of the Sri Lanka Department of Agriculture .7:95-102
  3. K.K.S.Fernando, J.H.B.H. Bandara and R.M.T. Rajapakse. 2005.Selection of rice varieties for gene pyramiding for Bacterial leaf Blight disease resistance. Proceeding of the 25th Annual Session, Institute of Biology, Sri Lanka.pp28
  4. K.K.S. Fernando and D.M.K.K. Dissanayake.2005. Molecular analysis of Asian rice Gall Midge, Orseolia oryzae (Wood-Mason), biotypes in Sri Lanka. Proceeding of the 25th Annual Session, Institute of Biology, Sri Lanka. pp 32.

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Last updated: July 10, 2007